Disrupting GluA2 phosphorylation potentiates reinstatement of cocaine seeking

NEUROPHARMACOLOGY

卷:111

Article

Briand, Lisa A.^1,2  Deutschmann, Andre U.¹  Ellis, Alexandra S.²  Fosnocht, Anne Q.¹ 

[1] Temple Univ, Dept Psychol, Weiss Hall,1701 North 13th St, Philadelphia, PA 19122 USA

[2] Temple Univ, Neurosci Program, Philadelphia, PA 19122 USA

关键词: Cocaine addiction;    Reinstatement;    AMPA trafficking;    Self-administration;    Conditioned place preference;   

DOI  :  10.1016/j.neuropharm.2016.09.010

来源: Elsevier

【 摘 要 】

Addiction is associated with changes in synaptic plasticity mediated, in part, by alterations in the trafficking and stabilization of AMPA receptors at synapses within the nucleus accumbens. Exposure to cocaine can lead to protein kinase C-mediated phosphorylation of GluA2 AMPA subunits and this phosphorylation event leads to the internalization of GluA2-containing AMPARs, which are calcium impermeable. However, it is not clear whether this internalization is necessary for the expression of addictive phenotypes. Utilizing a mouse with a point mutation within the GluA2 subunit c-terminus, the current study demonstrates that disrupting PKC-mediated GluA2 phosphorylation potentiates reinstatement of both cue-induced cocaine seeking and cocaine conditioned reward without affecting operant learning, food self-administration or cocaine sensitization. Electrophysiological recordings revealed increased GluA2-mediated AMPA transmission as evidenced by increased sEPSC amplitude without any changes in sEPSC frequency or rectification. In support of this increase in GluA2 activity mediating the augmented cocaine reinstatement, we found that accumbal overexpression of GluA2 recapitulated this behavioral effect in wildtype mice while not altering reinstatement behavior in the GluA2 K882A knock in mice. In addition, disrupting GluA2 phosphorylation was associated with blunted long-term depression in the nucleus accumbens, mimicking the anaplasticity seen following cocaine self-administration. Taken together these results indicate that disrupting GluA2 phosphorylation and increasing GluA2-mediated transmission in the nucleus accumbens leads to increased vulnerability to cocaine relapse. Further, these results indicate that modulating GluA2-containing AMPAR trafficking can contribute to addictive phenotypes in the absence of alterations in GluA2-lacking receptors. These results highlight the GluA2 phosphorylation site as a novel target for the development of cocaine addiction therapeutics. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

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