Delayed administration of the human anti-RGMa monoclonal antibody elezanumab promotes functional recovery including spontaneous voiding after spinal cord injury in rats

NEUROBIOLOGY OF DISEASE

卷:143

Article

Mothe, Andrea J.¹  Coelho, Marlon¹  Huang, Lili²  Monnier, Philippe P.^1,5  Cui, Yi-Fang³  Mueller, Bernhard K.^3,7  Jacobson, Peer B.⁴  Tator, Charles H.^1,6 

[1] Univ Hlth Network, Toronto Western Hosp, Krembil Brain Inst, Krembil Discovery Tower,60 Leonard Ave, Toronto, ON M5T 0S8, Canada

[2] AbbVie Biores Ctr, Worcester, MA 01605 USA

[3] AbbVie Deutschland GmbH & Co KG, Neurosci Res, Knollstr, D-67061 Ludwigshafen, Germany

[4] AbbVie, Integrated Sci & Technol, N Chicago, IL 60064 USA

[5] Univ Toronto, Dept Ophthalmol & Vis Sci, Toronto, ON M5S 3H6, Canada

[6] Univ Toronto, Dept Surg, Div Neurosurg, Toronto, ON M5T 2S8, Canada

[7] BMCO, D-30657 Hannover, Germany

关键词: Repulsive Guidance Molecule A (RGMa);    Spinal cord injury;    Clip impact-compression;    Neuronal survival;    Axonal plasticity;    Bladder function;    Elezanumab;    ABT-555;   

DOI  :  10.1016/j.nbd.2020.104995

来源: Elsevier

【 摘 要 】

Spinal cord injury (SCI) often results in permanent functional loss due to a series of degenerative events including cell death, axonal damage, and the upregulation of inhibitory proteins that impede regeneration. Repulsive Guidance Molecule A (RGMa) is a potent inhibitor of axonal growth that is rapidly upregulated following injury in both the rodent and human central nervous system (CNS). Previously, we showed that monoclonal antibodies that specifically block inhibitory RGMa signaling promote neuroprotective and regenerative effects when administered acutely in a clinically relevant rat model of thoracic SCI. However, it is unknown whether systemic administration of RGMa blocking antibodies are effective for SCI after delayed administration. Here, we administered elezanumab, a human monoclonal antibody targeting RGMa, intravenously either acutely or at 3 h or 24 h following thoracic clip impact-compression SCI. Rats treated with elezanumab acutely and at 3 h post-injury showed improvements in overground locomotion and fine motor function and gait. Rats treated 24 h post-SCI trended towards better recovery demonstrating significantly greater stride length and swing speed. Treated rats also showed greater tissue preservation with reduced lesion areas. As seen with acute treatment, delayed administration of elezanumab at 3 h post-SCI also increased perilesional neuronal sparing and serotonergic and corticospinal axonal plasticity. In addition, all elezanumab treated rats showed earlier spontaneous voiding ability and less post-trauma bladder wall hypertrophy. Together, our data demonstrate the therapeutic efficacy of delayed systemic administration of elezanumab in a rat model of SCI, and uncovers a new role for RGMa inhibition in bladder recovery following SCI.