Immune transcriptome alterations in the temporal cortex of subjects with autism

NEUROBIOLOGY OF DISEASE

卷:30

Article

Garbett, Krassimira¹  Ebert, Philip J.¹  Mitchell, Amanda¹  Lintas, Carla^2,3  Manzi, Barbara⁴  Mirnics, Karoly^1,5  Persico, Antonio M.^2,3 

[1] Vanderbilt Univ, Dept Psychiat, Nashville, TN 37203 USA

[2] Univ Campus Biomed, Lab Mol Psychiat & Neurogenet, Rome, Italy

[3] IRCCS Fdn Santa Lucia, Dept Expt Neurosci, Lab Mol Psychiat & Psychiat Genet, Rome, Italy

[4] Univ Roma Tor Vergata, Dept Child Neuropsychiat, Rome, Italy

[5] Vanderbilt Univ, Vanderbilt Kennedy Ctr Res Human Dev, Nashville, TN 37203 USA

关键词: DNA microarray;    gene expression;    transcriptoine;    autism;    qPCR;    post mortem;    temporal cortex;   

DOI  :  10.1016/j.nbd.2008.01.012

来源: Elsevier

【 摘 要 】

Autism is a severe disorder that involves both genetic and environmental factors. Expression profiling of the superior temporal gyrus of six autistic subjects and matched controls revealed increased transcript levels of many immune system-related genes. We also noticed changes in transcripts related to cell communication, differentiation, cell cycle regulation and chaperone systems. Critical expression changes were confirmed by qPCR (BCL6, CHI3L1, CYR61, IF116, IFITM3, MAP2K3, PTDSR, RFX4, SPP1, RELN, NOTCH2, RIT1, SFN, GADD45B, HSPA6, HSPB8 and SERPINH1). Overall, these expression patterns appear to be more associated with the late recovery phase of autoimmune brain disorders, than with the innate immune response characteristic of neurodegenerative diseases. Moreover, a variance-based analysis revealed much greater transcript variability in brains from autistic subjects compared to the control group, suggesting that these genes may represent autism susceptibility genes and should be assessed in follow-up genetic studies. (C) 2008 Elsevier Inc. All rights reserved.

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