Behavioral defects associated with amygdala and cortical dysfunction in mice with seeded α-synuclein inclusions

NEUROBIOLOGY OF DISEASE

卷:134

Article

Stoyka, Lindsay E.¹  Arrant, Andrew E.¹  Thrasher, Drake R.¹  Russell, Dreson L.¹  Freire, Jennifer¹  Mahoney, Casey L.¹  Narayanan, Ashwin¹  Dib, Aseel G.¹  Standaert, David G.¹  Volpicelli-Daley, Laura A.¹ 

[1] Univ Alabama Birmingham, Ctr Neurodegenerat & Expt Therapeut, 1719 6th Ave South, Birmingham, AL 35294 USA

关键词: Dementia with Lewy Bodies;    Parkinson's disease dementia;    Alpha-synuclein;    Fear conditioning;    Social dominance;    Fibrils;    Cortex;    Amygdala;    Lewy neurites;    Lewy bodies;    Excitatory neurons;   

DOI  :  10.1016/j.nbd.2019.104708

来源: Elsevier

【 摘 要 】

Parkinson's disease (PD) is defined by motor symptoms such as tremor at rest, bradykinesia, postural instability, and stiffness. In addition to the classical motor defects that define PD, up to 80% of patients experience cognitive changes and psychiatric disturbances, referred to as PD dementia (PDD). Pathologically, PD is characterized by loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) and intracellular inclusions, called Lewy bodies and Lewy neurites, composed mostly of alpha-synuclein. Much of PD research has focused on the role of alpha-synuclein aggregates in degeneration of SNpc dopamine neurons because of the impact of loss of striatal dopamine on the classical motor phenotypes. However, abundant Lewy pathology is also found in other brain regions including the cortex and limbic brain regions such as the amygdala, which may contribute to non-motor phenotypes. Little is known about the consequences of alpha-synuclein inclusions in these brain regions, or in neuronal subtypes other than dopamine neurons. This project expands knowledge on how alpha-synuclein inclusions disrupt behavior, specifically non-motor symptoms of synucleinopathies. We show that bilateral injections of fibrils into the striatum results in robust bilateral alpha-synuclein inclusion formation in the cortex and amygdala. Inclusions in the amygdala and prefrontal cortex primarily localize to excitatory neurons, but unbiased stereology shows no significant loss of neurons in the amygdala or cortex. Fibril injected mice show defects in a social dominance behavioral task and fear conditioning, tasks that are associated with prefrontal cortex and amygdala function. Together, these observations suggest that seeded a-synuclein inclusion formation impairs behaviors associated with cortical and amygdala function, without causing cell loss, in brain areas that may play important roles in the complex cognitive features of PDD.