Age and α-synuclein expression interact to reveal a dependence axons on endogenous Akt/PKB signaling

NEUROBIOLOGY OF DISEASE

卷:44

Article

Kim, Sang Ryong¹  Ries, Vincent¹  Cheng, Hsiao-Chun¹  Kareva, Tatyana¹  Oo, Tinmarla F.¹  Yu, W. Haung^2,3  Duff, Karen^2,3  Kholodilov, Nikolai¹  Burke, Robert E.¹ 

[1] Columbia Univ, Dept Neurol, New York, NY 10032 USA

[2] Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10032 USA

[3] Columbia Univ, Taub Inst, New York, NY 10032 USA

关键词: Axon;    Akt;    Substantia nigra;    Dopamine;    Parkinson's disease;    Synuclein;   

DOI  :  10.1016/j.nbd.2011.07.003

来源: Elsevier

【 摘 要 】

The mechanisms underlying the chronic neurodegeneration that occurs in Parkinson's disease (PD) are unknown. One emerging hypothesis is that neural systems deteriorate and eventually degenerate due to a primary failure of either extrinsic neurotrophic support or the intrinsic cellular pathways that mediate such support. One of the cellular pathways that have been often identified in mediating neurotrophic effects is that of PI3K/Akt signaling. In addition, recent observations have suggested a primary failure of PI3K/Akt signaling in animal models and in PD patients. Therefore, to explore the possible role of endogenous Akt signaling in maintaining the viability and functionality of substantia nigra (SN) dopamine neurons, one of the principal systems affected in PD, we have used an adeno-associated viral vector to transduce them with a dominant negative (ON) form of Akt, the pleckstrin homology (PH) domain alone (DN(PH)-Akt). In addition, we have examined the effect of DN(PH)-Akt in murine models of two risk factors for human PD: advanced age and increased expression of a-synuclein. We find that transduction of these neurons in normal adult mice has no effect on any aspect of their morphology at 4 or 7 weeks. However, in both aged mice and in transgenic mice with increased expression of human a-synuclein we observe decreased phenotypic expression of the catecholamine synthetic enzyme tyrosine hydroxylase (TH) in dopaminergic axons and terminals in the striatum. In aged transgenic a-synuclein over-expressing mice this reduction was 2-fold as great. We conclude that the two principal risk factors for human PD, advanced age and increased expression of a-synuclein, reveal a dependence of dopaminergic neurons on endogenous Akt signaling for maintenance of axonal phenotype. (C) 2011 Elsevier Inc. All rights reserved.

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