In vitro ictogenesis and parahippocampal networks in a rodent model of temporal lobe epilepsy

NEUROBIOLOGY OF DISEASE

卷:39

Article

Panuccio, G.^1,2  D'Antuono, M.^1,2  de Guzman, P.^1,2  De Lannoy, L.^1,2  Biagini, G.³  Avoli, M.^1,2,4 

[1] McGill Univ, Montreal Neurol Inst, Montreal, PQ H3A 2B4, Canada

[2] McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ H3A 2B4, Canada

[3] Univ Modena, Dipartimento Sci Biomed, I-41100 Modena, Italy

[4] Univ Roma La Sapienza, Dipartimento Med Sperimentale, I-00185 Rome, Italy

关键词: 4-Aminopyridine;    Amygdala;    CA3;    Hippocampus;    Ictogenesis;    Pilocarpine;    Subiculum;    Temporal lobe epilepsy;   

DOI  :  10.1016/j.nbd.2010.05.003

来源: Elsevier

【 摘 要 】

Temporal lobe epilepsy (TLE) is a chronic epileptic disorder involving the hippocampal formation. Details on the interactions between the hippocampus proper and parahippocampal networks during ictogenesis remain, however, unclear. In addition, recent findings have shown that epileptic limbic networks maintained in vitro are paradoxically less responsive than non-epileptic control (NEC) tissue to application of the convulsant drug 4-aminopyridine (4AP). Field potential recordings allowed us to establish here the effects of 4AP in brain slices obtained from NEC and pilocarpine-treated epileptic rats; these slices included the hippocampus and parahippocampal areas such as entorhinal and perirhinal cortices and the amygdala. First, we found that both types of tissue generate epileptiform discharges with similar electrographic characteristics. Further investigation showed that generation of robust ictal-like discharges in the epileptic rat tissue is (i) favored by decreased hippocampal output (ii) reinforced by EC-subiculum interactions and (iii) predominantly driven by amygdala networks. We propose that a functional switch to alternative synaptic routes may promote network hyperexcitability in the epileptic limbic system. (C) 2010 Elsevier Inc. All rights reserved.

【 授权许可】

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