Impact of transient acute hypoxia on the developing mouse EEG

NEUROBIOLOGY OF DISEASE

卷:68

Article

Zanelli, S.¹  Goodkin, H. P.^1,3  Kowalski, S.²  Kapur, J.^2,3 

[1] Univ Virginia, Dept Pediat, Charlottesville, VA USA

[2] Univ Virginia, Dept Neurosci, Charlottesville, VA USA

[3] Univ Virginia, Dept Neurol, Charlottesville, VA USA

关键词: Neonatal;    Mouse;    EEG;    Hypoxia;    Reoxygenation;    Seizures;    Development;   

DOI  :  10.1016/j.nbd.2014.03.005

来源: Elsevier

【 摘 要 】

Hypoxemic events are common in sick preterm and term infants and represent the most common cause of seizures in the newborn period. Neonatal seizures often lack clinical correlates and are only recognized by electroencephalogram (EEG). The mechanisms leading from a hypoxic/ischemic insult to acute seizures in neonates remain poorly understood. Further, the effects of hypoxia on EEG at various developmental stages have not been fully characterized in neonatal animals, in part due to technical challenges. We evaluated the impact of hypoxia on neonatal mouse EEG to define periods of increased susceptibility to seizures during postnatal development. Hippocampal and cortical electrodes were implanted stereotaxically in C57BL/6 mice from postnatal age 3 (P3) to P15. Following recovery, EEG recordings were obtained during baseline, acute hypoxia (4% FiO(2) for 4 min) and reoxygenation. In baseline recordings, maturation of EEG was characterized by the appearance of a more continuous background pattern that replaced alternating high and low amplitude activity. Clinical seizures during hypoxia were observed more frequently in younger animals (100% P3-4, 87.5% P5-6, 93% P7-8, 83% P9-10, 33% P11-12, 17% P15, r(2) = 0.81) and also occurred at higher FiO(2) in younger animals (11.2 +/- 1.1% P3-P6 vs. 8.9 +/- 0.8% P7-12, p < 0.05). Background attenuation followed the initial hypoxemic seizure; progressive return to baseline during reoxygenation was observed in survivors. Electrographic seizures without clinical manifestations were observed during reoxygenation, again more commonly in younger animals (83% P3-4, 86% P5-6, 75% P7-8, 71% P9-10, 20% P11-12, r(2) = 0.82). All P15 animals died with this duration and degree of hypoxia. Post-ictal abnormalities included burst attenuation and post-anoxic myoclonus and were more commonly seen in older animals. In summary, neonatal mice exposed to brief and severe hypoxia followed by rapid reoxygenation reliably develop seizures and the response to hypoxia varies with postnatal age and maturation. (C) 2014 Published by Elsevier Inc.

【 授权许可】

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