Glutamate presynaptic vesicular transporter and postsynaptic receptor levels correlate with spatial memory status in aging rat models

NEUROBIOLOGY OF AGING

卷:36

Article

Menard, Caroline^1,2,3,4  Quirion, Remi^1,2  Vigneault, Erika¹  Bouchard, Sylvain⁵  Ferland, Guylaine^6,7  El Mestikawy, Salah^1,8  Gaudreau, Pierrette^3,4 

[1] Univ Montreal, Douglas Mental Hlth Univ Inst, Montreal, PQ H2X 0A9, Canada

[2] Univ Montreal, Dept Psychiat, Montreal, PQ H2X 0A9, Canada

[3] Univ Montreal, Ctr Hosp, Lab Neuroendocrinol Aging, Montreal, PQ H2X 0A9, Canada

[4] Univ Montreal, Dept Med, Montreal, PQ H3C 3J7, Canada

[5] Univ Montreal, Fac Med, Montreal, PQ H3C 3J7, Canada

[6] Univ Montreal, Inst Univ Geriatr Montreal, Res Ctr, Montreal, PQ, Canada

[7] Univ Montreal, Dept Nutr, Montreal, PQ H3C 3J7, Canada

[8] Univ Paris 06, CNRS, UMR7224, INSERM U952, Paris, France

关键词: Aging;    Spatial memory;    Glutamate receptors;    AMPA;    NMDA;    mGluR;    VGLUT;   

DOI  :  10.1016/j.neurobiolaging.2014.11.013

来源: Elsevier

【 摘 要 】

In humans, memory capacities are generally affected with aging, even without any reported neurologic disorders. The mechanisms behind cognitive decline are not well understood. We studied here whether postsynaptic glutamate receptor and presynaptic vesicular glutamate transporters (VGLUTs) levels may change in the course of aging and be related to cognitive abilities using various age-impaired (AI) or age-unimpaired rat strains. Twenty-four-month-old Long-Evans (LE) rats with intact spatial memory maintained postsynaptic ionotropic glutamate receptor levels in the hippocampal-adjacent cortex similar to those of young animals. In contrast, AI rats showed significantly reduced expression of ionotropic glutamate receptor GluR2, NR2A and NR2B subunits. In AI LE rats, VGLUT1 and VGLUT2 levels were increased and negatively correlated with receptor levels as shown by principal component analysis and correlation matrices. We also investigated whether glutamatergic receptors and VGLUT levels were altered in the obesity-resistant LOU/C/Jall (LOU) rat strain which is characterized by intact memory despite aging. No difference was observed between 24-month-old LOU rats and their young counterparts. Taken together, the unaltered spatial memory performance of 24-month-old age-unimpaired LE and LOU rats suggests that intact coordination of the presynaptic and postsynaptic hippocampal-adjacent cortex glutamatergic networks may be important for successful cognitive aging. Accordingly, altered expression of presynaptic and postsynaptic glutamatergic components, such as in AI LE rats, could be considered a marker of age-related cognitive deficits. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license.

【 授权许可】

Free