Age-related increase of sIAHP in prefrontal pyramidal cells of monkeys: relationship to cognition

NEUROBIOLOGY OF AGING

卷:33

Article

Luebke, J. I.¹  Amatrudo, J. M.¹ 

[1] Boston Univ, Sch Med, Dept Anat & Neurobiol, Boston, MA 02118 USA

关键词: Slice;    Patch-clamp;    Voltage-clamp;    Potassium channels;    Excitability;   

DOI  :  10.1016/j.neurobiolaging.2010.07.002

来源: Elsevier

【 摘 要 】

Reduced excitability, due to an increase in the slow afterhyperpolarization (and its underlying current sI(AHP)), occurs in CA1 pyramidal cells in aged cognitively-impaired, but not cognitively-unimpaired, rodents. We sought to determine whether similar age-related changes in the sI(AHP) occur in pyramidal cells in the rhesus monkey dorsolateral prefrontal cortex (dlPFC). Whole-cell patch-clamp recordings were obtained from layer 3 and layer 5 pyramidal cells in dlPFC slices prepared from young (9.6 +/- 0.7 years old) and aged (22.3 +/- 0.7 years old) behaviorally characterized subjects. The amplitude of the sI(AHP) was significantly greater in layer 3 (but not layer 5) cells from aged-impaired compared with both aged-unimpaired and young monkeys, which did not differ. Aged layer 3, but not layer 5, cells exhibited significantly increased action potential firing rates, but there was no relationship between sI(AH)P and firing rate. Thus, in monkey dlPFC layer 3 cells, an increase in sI(AHP) is associated with age-related cognitive decline; however, this increase is not associated with a reduction in excitability. (C) 2012 Elsevier Inc. All rights reserved.

【 授权许可】

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