Characterizing human vestibular sensory epithelia for experimental studies: new hair bundles on old tissue and implications for therapeutic interventions in ageing

NEUROBIOLOGY OF AGING

卷:36

Article

Taylor, Ruth R.¹  Jagger, Daniel J.¹  Saeed, Shakeel R.^1,2  Axon, Patrick³  Donnelly, Neil³  Tysome, James³  Moffatt, David³  Irving, Richard⁴  Monksfield, Peter⁴  Coulson, Chris⁴  Freeman, Simon R.^5,6  Lloyd, Simon K.^5,6  Forge, Andrew¹ 

[1] UCL Ear Inst, London WC1X 8EE, England

[2] UCLH NHS Fdn Trust, Royal Natl Throat Nose & Ear Hosp, London, England

[3] Univ Cambridge, Addenbrookes Hosp, NHS Fdn Trust, Cambridge CB2 2QQ, England

[4] Univ Hosp Birmingham, NHS Fdn Trust, Queen Elizabeth Med Ctr, Queen Elizabeth Hosp, Birmingham, W Midlands, England

[5] Cent Manchester Univ Hosp NHS Trust, Manchester Royal Infirm, Manchester, Lancs, England

[6] Salford Royal NHS Fdn Trust, Salford Royal Infirm, Salford, Lancs, England

关键词: Human vestibular;    Utricle;    Hair cells;    Supporting cells;    Stereocilia;    Aging pathologies;   

DOI  :  10.1016/j.neurobiolaging.2015.02.013

来源: Elsevier

【 摘 要 】

Balance disequilibrium is a significant contributor to falls in the elderly. The most common cause of balance dysfunction is loss of sensory cells from the vestibular sensory epithelia of the inner ear. However, inaccessibility of inner ear tissue in humans severely restricts possibilities for experimental manipulation to develop therapies to ameliorate this loss. We provide a structural and functional analysis of human vestibular sensory epithelia harvested at trans-labyrinthine surgery. We demonstrate the viability of the tissue and labeling with specific markers of hair cell function and of ion homeostasis in the epithelium. Samples obtained from the oldest patients revealed a significant loss of hair cells across the tissue surface, but we found immature hair bundles present in epithelia harvested from patients >60 years of age. These results suggest that the environment of the human vestibular sensory epithelium could be responsive to stimulation of developmental pathways to enhance hair cell regeneration, as has been demonstrated successfully in the vestibular organs of adult mice. (C) 2015 The Authors. Published by Elsevier Inc.

【 授权许可】

Free