Allopregnanolone restores hippocampal-dependent learning and memory and neural progenitor survival in aging 3xTgAD and nonTg mice

NEUROBIOLOGY OF AGING

卷:33

Article

Singh, Chanpreet²  Liu, Lifei²  Wang, Jun Ming³  Henry, Sherry³  Thompson, Richard F.²  Brinton, Roberta Diaz^1,2 

[1] Univ So Calif, Dept Pharmacol & Pharmaceut Sci, Pharmaceut Sci Ctr, Sch Pharm, Los Angeles, CA 90089 USA

[2] Univ So Calif, Neurosci Program, Los Angeles, CA 90089 USA

[3] Univ Mississippi, Med Ctr, Dept Pathol, Jackson, MS 39216 USA

关键词: Allopregnanolone;    Alzheimer's disease therapeutics;    Adult neurogenesis;    Trace eyeblink conditioning;    Mild cognitive impairment therapeutics;    Translational neuroscience;   

DOI  :  10.1016/j.neurobiolaging.2011.06.008

来源: Elsevier

【 摘 要 】

We previously demonstrated that allopregnanolone (AP alpha) increased proliferation of neural progenitor cells and reversed neurogenic and cognitive deficits prior to Alzheimer's disease (AD) pathology (Wang, J.M., Johnston, P. B., Ball, B. G., Brinton, R. D., 2005. The neurosteroid allopregnanolone promotes proliferation of rodent and human neural progenitor cells and regulates cell-cycle gene and protein expression. J. Neurosci. 25, 4706-4718; Wang, J. M., Singh, C., Liu, L., Irwin, R. W., Chen, S., Chung, E. J., Thompson, R. F., Brinton, R. D., 2010. Allopregnanolone reverses neurogenic and cognitive deficits in mouse model of Alzheimer's disease. Proc. Natl. Acad. Sci. U. S. A. 107, 6498-6503). Herein, we determined efficacy of AP alpha to restore neural progenitor cell survival and associative learning and memory subsequent to AD pathology in male 3xTgAD mice and their nontransgenic (nonTg) counterparts. AP alpha significantly increased survival of bromodeoxyuridine positive (BrdU+) cells and hippocampal-dependent associative learning and memory in 3xTgAD mice in the presence of intraneuronal amyloid beta (A beta) whereas AP alpha was ineffective subsequent to development of extraneuronal A beta plaques. Restoration of hippocampal-dependent associative learning was maximal by the first day and sustained throughout behavioral training. Learning and memory function in AP alpha-treated 3xTgAD mice was 100% greater than vehicle-treated and comparable to maximal normal nonTg performance. In aged 15-month-old nonTg mice, AP alpha significantly increased survival of bromodeoxyuridine-positive cells and hippocampal-dependent associative learning and memory. Results provide preclinical evidence that AP alpha promoted survival of newly generated cells and restored cognitive performance in the preplaque phase of AD pathology and in late-stage normal aging. (C) 2012 Elsevier Inc. All rights reserved.

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