Expression pattern of oxidative stress and antioxidant defense-related genes in the aging Fischer 344/NHsd rat cochlea

NEUROBIOLOGY OF AGING

卷:33

Article

Tanaka, Chiemi^1,2  Coling, Donald E.²  Manohar, Senthilvelan²  Chen, Guang-Di²  Hu, Bo Hua²  Salvi, Richard²  Henderson, Donald² 

[1] Oregon Hlth & Sci Univ, Oregon Hearing Res Ctr, Dept Otolaryngol, Portland, OR 97239 USA

[2] SUNY Buffalo, Ctr Hearing & Deafness, Buffalo, NY 14260 USA

关键词: Age-related hearing loss;    Presbycusis;    Cochlea;    Fischer 344;    Antioxidant;    Reactive oxygen species;    Oxidative stress;    Rat;    Genes;    Scd1;    Dhcr24;    Aass;    Cygb;    Duox2;    Gpx3;    Gpx6;    Gstk1;    Gsr;    Nqo1;    Slc38a5;    Txnip;    Vim;   

DOI  :  10.1016/j.neurobiolaging.2011.12.027

来源: Elsevier

【 摘 要 】

The biological mechanisms that give rise to age-related hearing loss (ARHL) are still poorly understood. However, there is growing recognition that oxidative stress may be an important factor. To address this issue, we measured the changes in the expression of cochlear oxidative stress and antioxidant defense-related genes in young (2 months old), middle-aged (12 months old), and old (21-25 months old) Fischer 344/NHsd (F344/NHsd) rats and compared gene expression changes with ARHL. A quantitative real-time reverse transcription polymerase chain reaction array revealed a significant age-related downregulation of only 1 gene, stearoyl-coenzyme A desaturase 1, and upregulation of 12 genes: 24-dehydrocholesterol reductase; aminoadipate-semialdehyde synthase; cytoglobin; dual oxidase 2; glutathione peroxidase 3; glutathione peroxidase 6; glutathione S-transferase, kappa 1; glutathione reductase; nicotinamide adenine dinucleotide phosphate (NAD(P) H) dehydrogenase, quinone 1; solute carrier Family 38, Member 5; thioredoxin interacting protein; and vimentin. Statistical analyses revealed significant correlations between gene expression and auditory function in 8 genes. Our results identified specific subsets of oxidative stress genes that appear to play an important role in ARHL in the Fischer 344/NHsd rat. (C) 2012 Elsevier Inc. All rights reserved.

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