Blastocoel volume is related to successful establishment of human embryonic stem cell lines

REPRODUCTIVE BIOMEDICINE ONLINE

卷:17

Article

Cheng, En-Hui^1,2  Chen, Wannhsin³  Chang, Shiun-Yin³  Huang, Jun-Jae³  Huang, Chun-Chia^1,4  Huang, Lii-Shung^1,4,5  Liu, Chung-Hsien⁶  Lee, Maw-Sheng^1,5,6,7 

[1] Lee Womens Hosp, Div Infertil, Taichung, Taiwan

[2] Chung Shan Med Univ, Inst Biochem & Biotechnol, Taichung, Taiwan

[3] Ind Technol Res Inst, Biomed Engn Res Labs, Hsinchu, Taiwan

[4] Cent Taiwan Univ Sci & Technol, Dept Biotechnol, Taichung, Taiwan

[5] Chung Shan Med Univ Hosp, Inst Med, Taichung, Taiwan

[6] Chung Shan Med Univ Hosp, Dept Obstet & Gynecol, Taichung, Taiwan

[7] China Med Univ, Dept Med, Taichung, Taiwan

关键词: blastocoel;    embryo;    human embryonic stem cells;    TWI;    whole embryo culture method;   

DOI  :  10.1016/S1472-6483(10)60229-5

来源: Elsevier

【 摘 要 】

Human embryonic stem cell (hESC) banks strive to establish hESC lines front discarded or surplus human embryos. The effect of embryo quality oil establishing hESC lines was investigated by observing Cultures derived front 28 Taiwanese fresh surplus and donated embryos that were cultured using the whole embryo method. Cultures of hESC lines were followed for 15 months. At the blastocyst stage, 14 of the 28 embryos were graded as good quality, defined as featuring it blastocoel volume of at least half of the embryo volume. Fourteen embryos did not meet these standards oil day 5. Five Successful hESC lines were derived front the good quality embryos (5/14: 35.7%): these hESC cells grew for 27-60 passages. In contrast, cells from poor quality embryos all stopped growing at the second or third passage. The Successful hESC exhibited typical stem cell characteristics, including the capacity for pluripotent differentiation. Embryo quality oil day 5. as defined by blastocoel Volume. is thus a strong predictor for Successful establishment of hESC lines.

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