Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos

REPRODUCTIVE BIOMEDICINE ONLINE

卷:24

Article

Chen, Chien-Hong¹  Chang, Wei-Fang¹  Liu, Chia-Chia¹  Su, Hwa-Yun¹  Shyue, Song-Kun³  Cheng, Winston T. K.¹  Chen, Y. Eugene⁴  Wu, Shinn-Chih¹  Du, Fuliang⁵  Sung, Li-Ying^1,2  Xu, Jie⁵ 

[1] Natl Taiwan Univ, Inst Biotechnol, Taipei 10764, Taiwan

[2] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan

[3] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan

[4] Univ Michigan, Med Ctr, Ctr Cardiovasc, Ann Arbor, MI USA

[5] Univ Maryland, TAP Program, Renova Life Inc, College Pk, MD 20742 USA

关键词: H4K5 acetylation;    inner cell mass;    Oct-4;    preimplantation;    rabbit;    trophectoderm;   

DOI  :  10.1016/j.rbmo.2012.01.001

来源: Elsevier

【 摘 要 】

Rabbit is a unique species to study human embryology; however, there are limited reports on the key transcription factors and epigenetic events of rabbit embryos. This study examined the Oct-4 and acetylated H4K5 (H4K5ac) patterns in rabbit embryos using immunochemistry staining. The average intensity of the Oct-4 signal in the nuclei of the whole embryo spiked upon fertilization, then decreased until the 8-cell stage and increased afterwards until the compact morula (CM) stage. It decreased thereafter from the CM stage to the early blastocyst (EB) stage, with a minimum at the expanded blastocyst (EXPB) stage and came back to a level similar to that of the CM-stage embryos in the hatching blastocysts (HB). The Oct-4 signal was observed in both the inner cell mass (ICM) and the trophectoderm (TE) cells of blastocysts. The average H4K5ac signal intensity of the whole embryo increased upon fertilization, started to decrease at the 4-cell stage, reached a minimum at the 8-cell stage, increased again at the EXPB stage and peaked at the HB stage. While TE cells maintained similar levels of H4K5ac throughout the blastocyst stages, ICM cells of HB showed higher levels of H4K5ac than those of EB and EXPB. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free