Characterization of a recurrent poor-quality embryo morphology phenotype and zygote transfer as a rescue strategy

REPRODUCTIVE BIOMEDICINE ONLINE

卷:24

Article

Sermondade, Nathalie^1,2  Delarouziere, Vanina^1,2  Ravel, Celia^1,2  Berthaut, Isabelle^1,2  Verstraete, Lieve³  Mathieu, Emmanuelle³  Antoine, Jean-Marie³  Mandelbaum, Jacqueline^1,2 

[1] Hop Tenon, APHP, Serv Histol & Biol Reprod, F-75970 Paris, France

[2] Univ Paris 06, ER9, Paris, France

[3] Hop Tenon, APHP, Serv Gynecol Obstet & Med Reprod, F-75970 Paris, France

关键词: embryo fragmentation;    IVF failure;    zygote transfer;   

DOI  :  10.1016/j.rbmo.2012.01.004

来源: Elsevier

【 摘 要 】

Some patients in IVF programmes repeatedly display an abnormal embryonic development characterized as soon as day 2 post fertilization by a high rate (>60%) of highly fragmented embryos (>= 40% of cytoplasmic fragments) leading to recurrent IVF failures. This study postulated that, for various maternal reasons, some embryos were unable to withstand the in-vitro environment and an early pronucleate-stage transfer was proposed to these couples. Fifty-three patients with recurrent IVF failures (a mean of 2.8 +/- 1.0 previous attempts) characterized by low embryonic quality (a mean of 62.7% of the embryos with extended fragmentation) were included this transfer protocol. As in previous cycles, the mean number of oocytes retrieved and the fertilization rate were normal. The mean number of zygotes per transfer was 2.24. Fourteen clinical pregnancies were obtained, representing a pregnancy rate and a delivery rate per oocyte retrieval of 26.4% and 18.9%, respectively. Recurrent heavy and early embryo fragmentation in vitro characterizes around 3% of IVF couples and leads to lack of transfer or implantation failure. These data on fresh zygote transfers are encouraging and may provide a valid alternative solution for some of these patients. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free