Elucidating nuclear reprogramming mechanisms: taking a synergistic approach

REPRODUCTIVE BIOMEDICINE ONLINE

卷:16

Article; Proceedings Paper

Sullivan, Stephen^1,2  Ichida, Justin K.^1,2  Umezawa, Akihiro³  Akutsu, Hidenori³ 

[1] Harvard Univ, Dept Cellular & Mol Biol, Stowers Med Inst, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Cellular & Mol Biol, Harvard Stem Cell Inst, Cambridge, MA 02138 USA

[3] Natl Res Inst Child Hlth & Dev, Dept Reprod Biol & Pathol, Setagaya Ku, Tokyo 1578535, Japan

关键词: developmental potential;    differentiation;    embryonic stem cell;    nuclear reprogramming;    nuclear transfer;    pluripotency;   

DOI  :  10.1016/S1472-6483(10)60555-X

来源: Elsevier

【 摘 要 】

Nuclear reprogramming is the process by which a differentiated somatic nucleus has developmental potential restored to it. It involves heritable changes in gene expression as well as structural and functional changes to chromatin structure. This process is naturally induced immediately after fertilization, but can also be artificially induced by nuclear transfer, cell fusion and also now by viral transduction with four stem cell genes. However, the frequency of successful reprogramming is low in each system. The highest success rates, those using nuclear transfer, are only of the order of 2-5%. This article briefly reviews these three methods and proposes a synergistic approach where conditions that facilitate reprogramming in one system are transposed to the others. This might increase the incidence of successful reprogramming and identify common steps necessary for the reacquisition of developmental potential.

【 授权许可】

Free