Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes

REPRODUCTIVE BIOMEDICINE ONLINE

卷:23

Article

Lin, Yu-Hung^1,2,3,4  Hwang, Jiann-Loung^1,4  Seow, Kok-Min^1,5  Huang, Lee-Wen^1,2  Hsieh, Bih-Chwen^1,2  Chen, Hen-Ju^1,2  Tzeng, Chii-Ruey⁴  Bai, Chyi-Huey⁶ 

[1] Shin Kong Wu Ho Su Mem Hosp, Dept Obstet & Gynecol, Taipei, Taiwan

[2] Fu Jen Catholic Univ, Sch Med, Taipei, Taiwan

[3] Natl Taiwan Univ Hosp, Dept Obstet & Gynecol, Taipei, Taiwan

[4] Taipei Med Univ, Dept Obstet & Gynecol, Taipei, Taiwan

[5] Natl Yang Ming Univ, Dept Obstet & Gynecol, Taipei 112, Taiwan

[6] Taipei Med Univ, Sch Publ Hlth, Taipei, Taiwan

关键词: BMP-15;    c-AMP;    FSH;    GDF-9;    in-vitro maturation;   

DOI  :  10.1016/j.rbmo.2011.03.019

来源: Elsevier

【 摘 要 】

The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development. (C) 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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