Perivitelline threads associated with fragments in human cleavage stage embryos observed through time-lapse microscopy

REPRODUCTIVE BIOMEDICINE ONLINE

卷:35

Article

Derrick, Ranya^1,2  Hickman, Cristina^1,2  Oliana, Oriol¹  Wilkinson, Thomas¹  Gwinnett, Danielle¹  Whyte, Lucy Benham³  Carby, Anna¹  Lavery, Stuart^1,2 

[1] Boston Pl Clin, Fertil Partnership, 20 Boston Pl, London NW1 6ER, England

[2] Imperial Coll, Hammersmith Campus, London W12 0NN, England

[3] Univ Oxford, Med Sci Grad Sch, Oxford OX3 9DU, England

关键词: corona radiata;    fragmentation;    IVF;    morphokinetics;    perivitelline threads;    time-lapse;   

DOI  :  10.1016/j.rbmo.2017.08.026

来源: Elsevier

【 摘 要 】

Perivitelline threads (PVT) are defined as thin filaments that extend across the perivitelline space connecting the zona pellucida with the oolemma or, in some cases, blastomere membrane. This is the first report of PVT in human embryos. Time-lapse imagery from 525 blastocysts with either tested ploidy, known implantation status, or both, were reviewed for the presence of PVT, the cell stage when PVT were first observed, association with fragmentation, ploidy or implantation potential; PVT were observed in most embryos (404/525 [77%]). The euploidy rate was similar in embryos with PVT (61/152 [40%]) and without PVT (17/35 [49%]). Implantation rates were also similar in embryos with PVT (64/259 [25%]) and without PVT (25/90 [28%]). In the embryos in which PVT were observed, 98% (396/404) developed at the two-cell stage. In most embryos (384/404 [95%]), PVT were observed to directly pull fragments from the embryo. Fragmentation occurred significantly less frequently in embryos without PVT compared with PVT (81/121 [67%] versus 388/404 [96%]; P < 0.001). These data suggest an association between PVT and fragmentation. This study is limited in that PVT were not characterized so their nature and origin remain unknown and to be determined in future studies. (C) 2017 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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