Comparison of four media types during 3-day human IVF embryo culture

REPRODUCTIVE BIOMEDICINE ONLINE

卷:10

Article

Aoki, VW ; Wilcox, AL ; Peterson, CM ; Parker-Jones, K ; Hatasaka, HH ; Gibson, M ; Huang, I ; Carrell, DT

关键词: comparison;    culture;    embryo;    human;    IVF;    media;   

DOI  :  10.1016/S1472-6483(10)61667-7

来源: Elsevier

【 摘 要 】

The aim of this study was to compare the effectiveness of human tubal fluid (HTF), G1.2 (R), Sage Cleavageo (R) and Life Global (R) media for IVF outcome during 3-day culture of human embryos. A three-phase auto-controlled study was conducted in which IVF outcome was compared between (1) HTF and G 1.2, (2) HTF and Cleavage, and (3) Cleavage and Life Global. In phase 1, no differences in embryo quality were observed between HTF and G 1.2. However, embryos derived from intracytoplasmic sperm injection (ICSI) displayed significantly improved quality when grown in HTF versus G1.2. No differences in pregnancy and implantation rates were observed in cases where embryos transferred were grown exclusively in HTF or G 1.2 media. In phase 2, embryo quality was significantly improved for embryos cultured in Cleavage versus HTF media (P < 0.00 1). However, pregnancy, implantation and spontaneous abortion rates were similar between the two media. In phase 3, there were no differences in embryo quality, pregnancy, implantation, and spontaneous abortion rates between Cleavage and Life Global media. Overall, the data indicate that Life Global and Cleavage media yield similar results in a 3-day IVF culture programme. Cleavage medium is superior to HTF, as evidenced by significantly improved embryo quality (P < 0.001). Meanwhile, HTF medium is superior to G1.2 for ICSI cases.

【 授权许可】

Free