Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles

REPRODUCTIVE BIOMEDICINE ONLINE

卷:17

Article

Vanderzwalmen, Pierre^1,2,3  Hiemer, Antje¹  Rubner, Paul¹  Bach, Magnus¹  Neyer, Anton¹  Stecher, Astrid¹  Uher, Petr²  Zintz, Martin¹  Lejeune, Bernard³  Vanderzwalmen, Sabine³  Cassuto, Guy⁴  Zech, Nicolas H.^5,6 

[1] Inst Reprod Med & Endocrinol, Bregenz, Austria

[2] Inst Reprod Med & Endocrinol, Plzen, Czech Republic

[3] Ctr Hosp Inter Reg Cavell CHIREC, Brussels, Belgium

[4] Lab Drouot, Paris, France

[5] Illinois Masonic Med Ctr, Inst Reprod Genet, Chicago, IL 60657 USA

[6] Univ Zurich Hosp, Dept Obstet, Zurich, Switzerland

关键词: blastocyst;    ICSI;    IMSI;    sperm nuclei;    vacuole;   

DOI  :  10.1016/S1472-6483(10)60308-2

来源: Elsevier

【 摘 要 】

Spermatozoa selection at high magnification before intracytoplasmic sperm injection seems to be positively associated with pregnancy rates after day 3 embryo transfers. The aim was to demonstrate an association between the presence of vacuoles in sperm nuclei and the competence of embryos to develop to day 5. Grading of spermatozoa at x6000-x12,500 magnification: grade I, no vacuoles; grade II, <= 2 small vacuoles; grade III, >= 1 large vacuole; grade IV, large vacuoles with other abnormalities. The outcome of embryo development in a group of 25 patients after sibling oocyte injection with the four different grades of spermatozoa showed no significant difference in embryo quality up to day 3. However, the occurrence of blastocyst formation was 56.3 and 61.4% with grade I and It spermatozoa respectively, compared with 5.1% with grade III and 0% with grade IV respectively (P < 0.001). Spermatozoa selection at high magnification using Nomarski interference contrast is useful to identify more precisely the size and the number of nuclear vacuoles that greatly exert a negative effect on embryo development to the blastocyst stage. These observations confirm previous studies pointing to possible 'early and late paternal effects', both of which may have an impact on early embryonic development.

【 授权许可】

Free