Role of PTGS2-generated PGE2 during gonadotrophin-induced bovine oocyte maturation and cumulus cell expansion

REPRODUCTIVE BIOMEDICINE ONLINE

卷:28

Article

Marei, Waleed F.^1,2  Abayasekara, D. Robert E.¹  Wathes, D. Claire¹  Fouladi-Nashta, Ali A.¹ 

[1] Royal Vet Coll, Dept Comparat Biomed Sci, Reprod Genes & Dev Res Grp, Hatfield AL9 7TA, Herts, England

[2] Cairo Univ, Fac Vet Med, Dept Theriogenol, Giza 12211, Egypt

关键词: AH6809;    COX2;    NS398;    oocytes;    PGE(2);    siRNA;   

DOI  :  10.1016/j.rbmo.2013.11.005

来源: Elsevier

【 摘 要 】

Prostaglandin E-2 (PGE(2)) is an autocrine/paracrine factor which mediates gonadotrophin (Gn) stimulation of cumulus expansion and oocyte maturation in rodents. Its role in bovine oocyte maturation is less characterized. This study detected PTGS2 (COX2) and PGE synthases (PTGES1, PTGES2 and PTGES3) in bovine cumulus-oocyte complexes (COC). Only PTGS2 and PTGES1 expression changed during maturation. In Gn-free media, no cumulus expansion and similar to 45% nuclear maturation was achieved, while Gn-induced maturation showed full cumulus expansion (score 3) and similar to 87% maturation. PGE(2) supplementation without Gn induced mild cumulus expansion (score 0.5-1) but increased nuclear maturation to levels similar to those obtained with Gn alone. In the presence of Gn, exogenous PGE(2) did not affect expansion or nuclear maturation and subsequent embryo development. Treatment with PTGS2 selective inhibitor (NS398), PTGS2-specific siRNA or PTGER2-receptor antagonist (AH6809) resulted in similar to 20-25% reduction in nuclear maturation. NS398 and AH6809 did not affect cumulus expansion. Most oocytes not reaching metaphase of second meiosis (MII) following NS398, AH6809 and PTGS2-specific siRNA treatments were at MI. After longer maturation, NS398-treated oocytes had normal MII rate and uncompromised embryo development. PGE(2) has a limited role in cumulus expansion in bovine COC but is important for the timing of Gn-induced nuclear maturation. (C) 2013, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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