Outcomes of immature oocytes collected from ovarian tissue for cryopreservation in adult and prepubertal patients

REPRODUCTIVE BIOMEDICINE ONLINE

卷:34

Article

Fasano, Giovanna^1,2  Dechene, Julie¹  Antonacci, Raffaella²  Biramane, Jamila²  Vannin, Anne-Sophie²  Van Langendonckt, Anne²  Devreker, Fabienne²  Demeestere, Isabelle^1,2 

[1] Univ Libre Bruxelles, Res Lab Human Reprod, Campus Erasme, Brussels, Belgium

[2] Univ Libre Bruxelles, Dept Obstet & Gynecol, Fertil Clin, CUB Erasme Hosp, Brussels, Belgium

关键词: Cryobanking;    Fertility preservation;    In-vitro maturation;    Oocyte;    Ovarian tissue;    Vitrification;   

DOI  :  10.1016/j.rbmo.2017.03.007

来源: Elsevier

【 摘 要 】

The efficiency of oocyte in-vitro maturation (IVM) and vitrification procedures after ex-vivo collection from ovarian tissue were assessed according to patient age, number of retrieved oocytes and tissue transport conditions. The combined procedure was performed in 136 patients: 130 adults (mean 27.6 +/- 5.6 years) and six prepubertal girls (mean 8.7 +/- 2.3 years). A higher mean number of oocytes were collected in girls compared with adults 111.5 +/- 8.0 versus 3.8 +/- 4.2, respectively, P < 0.0011 but the percentage of degenerated oocytes was significantly higher in girls 135.5% versus 17.1%, respectively, P<0.0011. IVM rates were significantly lower in prepubertal than postpubertal population 110.3% versus 28.1%, P=0.0021. In adults, a negative correlation was observed between number of retrieved oocytes and age (P = 0.002; r = -0.271); the correlation was positive between anti-Mullerian hormone (AMH) and number of collected oocytes (P = 0.002; r = 0.264). IVM rates were not correlated with AMH levels (r = 0.06) or age (r = -0.033). At present, nine oocytes and one embryo have been warmed in four patients and one biochemical pregnancy obtained. This suggests the combined procedure could be an additional option for fertility preservation. (C) 2017 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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