Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos

REPRODUCTIVE BIOMEDICINE ONLINE

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Article

Chen, Chien-Hong¹  Xu, Jie³  Chang, Wei-Fang¹  Liu, Chia-Chia¹  Su, Hwa-Yun¹  Chen, Y. Eugene⁴  Du, Fuliang³  Sung, Li-Ying^1,2 

[1] Natl Taiwan Univ, Inst Biotechnol, Taipei 10764, Taiwan

[2] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan

[3] Univ Maryland, Renova Life Inc, TAP Program, College Pk, MD 20742 USA

[4] Univ Michigan, Med Ctr, Ctr Cardiovasc, Ann Arbor, MI USA

关键词: Cdx-2;    embryos;    H4K5ac;    in vivo;    Oct-4;    rabbit;   

DOI  :  10.1016/j.rbmo.2012.07.001

来源: Elsevier

【 摘 要 】

This study documents the spatial and temporal distribution of Oct-4, Cdx-2 and acetylated H4K5 (H4K5ac) by immunocytochemistry staining using in-vivo-derived rabbit embryos at different stages: day-3 compact morulae, day-4 early blastocysts, day-4 expanded blastocysts, day-5 blastocysts, day-6 blastocysts and day-7 blastocysts. The Oct-4 signal was stronger in the inner cell mass (ICM)/epiblast cells than in the trophectoderm (TE) cells in all blastocyst stages except day-4 expanded blastocysts, where the signal was similarly weak in both the ICM and TE cells. The Cdx-2 signal was first detected in a small number of TE cells of day-4 early blastocysts, and became evident in the TE cells exclusively afterwards. A consistently strong H4K5ac signal was observed in the TE cells in all blastocyst stages examined. In particular, this signal was stronger in the TE than in the ICM cells in day-4 early blastocysts, day-4 expanded blastocysts and day-5 blastocysts. Double staining of H4K5ac with either Oct-4 or Cdx-2 on embryos at different blastocyst stages confirmed these findings. This work suggests that day 4 is a critical timing for lineage formation in rabbit embryos. A combination of Oct-4, Cdx-2 and H4K5ac can be used as biomarkers to identify different lineage cells in rabbit blastocysts. RBM Online (c) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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