Aggregated chromosomes transfer in human oocytes

REPRODUCTIVE BIOMEDICINE ONLINE

卷:28

Article

Otsuki, Junko¹  Nagai, Yasushi¹  Sankai, Tadashi² 

[1] Nagai Clin, Saitama 3410004, Japan

[2] Natl Inst Biomed Innovat, Tsukuba Primate Res Ctr, Tsukuba, Ibaraki, Japan

关键词: aggregated chromosomes;    aneuploidy;    heteroplasmy;    microinjection;    mitochondrial disease;    nuclear transfer;   

DOI  :  10.1016/j.rbmo.2013.10.024

来源: Elsevier

【 摘 要 】

Germinal-vesicle (GV) transfer, spindle-chromosome complex transfer in metaphase-II oocytes and two pronuclei transfer have been evaluated as possible treatments for patients who have mitochondrial diseases. However, GV transfers often lead to heteroplasmy while the other two methods are frequently associated with aneuploidy. The present study used a new method based on the transfer of aggregated chromosomes, which occurs in human oocytes, before the metaphase spindle is established. (C) 2013, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free