Quantitative analysis of follistatin (FST) promoter methylation in peripheral blood of patients with polycystic ovary syndrome

REPRODUCTIVE BIOMEDICINE ONLINE

卷:26

Article

Sang, Qing^1,2,3  Zhang, Shaozhen⁵  Zou, Shien⁶  Wang, Huan^1,2,3  Feng, Ruizhi^1,2,3  Li, Qiaoli³  Jin, Li^1,2  He, Lin^3,4  Xing, Qinghe^3,4  Wang, Lei^1,2,3,4 

[1] Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

[2] Fudan Univ, Sch Life Sci, MOE Key Lab Contemporary Anthropol, Shanghai 200433, Peoples R China

[3] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

[4] Shanghai Jiao Tong Univ, Minist Educ, Bio X Ctr, Key Lab Genet Dev & Neuropsychiat Disorders, Shanghai 200030, Peoples R China

[5] Shanghai Ninth Hosp, Shanghai, Peoples R China

[6] Fudan Univ, Dept Gynecol Obstet & Gynecol, Shanghai 200433, Peoples R China

关键词: follistatin;    FST;    PCOS;    quantitative methylation analysis;   

DOI  :  10.1016/j.rbmo.2012.10.011

来源: Elsevier

【 摘 要 】

Epigenetic mechanisms may contribute to polycystic ovary syndrome (PCOS). To date, however, no studies have associated CpG methylation levels of any candidate gene with PCOS susceptibility. Follistatin (FST), an activin-binding protein, is expressed in numerous tissues and is shown to have linkage with PCOS. However, results from case-control association analyses between this gene and PCOS are inconsistent. Thus, this study investigated possible association of methylation levels in the promoter and 5'-untranscribed region (UTR) of the FST gene with PCOS incidence in peripheral blood leukocytes and endometrial tissue. Using mass array quantitative methylation analysis, first the 5'-UTR methylation in FST was analysed in 130 PCOS patients and 120 controls. The methylation level of the FST gene was further studied in endometrium from 24 controls and 24 PCOS patients. This study demonstrates that methylation levels of CpG sites in the FST promoter and 5'-UTR are not associated with PCOS. Nonetheless, this was the first study to quantitatively evaluate the methylation levels of a candidate gene in association with PCOS. Further studies should be performed to examine methylation in other candidate genes. Understanding the epigenetic mechanisms involved in PCOS may yield new insights into the pathophysiology of the disorder. RBMOnline (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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