DNA methylation and histone post-translational modifications in the mouse germline following in-vitro maturation of fresh or cryopreserved prepubertal testicular tissue

REPRODUCTIVE BIOMEDICINE ONLINE

卷:39

Article

Oblette, Antoine¹  Rondeaux, Julie¹  Dumont, Ludovic¹  Delessard, Marion¹  Saulnier, Justine¹  Rives, Aurelie¹  Rives, Nathalie¹  Rondanino, Christine¹ 

[1] Normandie Univ, UNIROUEN, EA 4308 Gametogenesis & Gamete Qual, Rouen Univ Hosp,Dept Reprod Biol CECOS, F-76000 Rouen, France

关键词: Cryopreservation protocols;    DNA methylation;    Epigenetic modification;    Histone post-translational modifications;    In-vitro spermatogenesis;    Mouse prepubertal testis;   

DOI  :  10.1016/j.rbmo.2019.05.007

来源: Elsevier

【 摘 要 】

Research question: Do cryopreservation and in-vitro culture procedures affect the expression of DNA methyltransferases (DNMT) and histone-modifying enzymes, as well as the establishment of DNA methylation and histone post-translational modifications (PTM) in germ cells in prepubertal mouse testicular tissue? Design: This study investigated the expression of epigenetic modification enzymes, DNA methylation and histone PTM, and the spermatogenic progression after in-vitro maturation of fresh or cryopreserved mouse prepubertal testicular tissue. Fresh or cryopreserved testicular fragments from 6-7 days post-partum mice were cultured for 30 days in the presence of retinol with or without FSH. Results: The in-vitro maturation of fresh or cryopreserved tissue allowed the differentiation of spermatogonia into spermatozoa. Differences in the levels of transcripts encoding epigenetic modification enzymes (Dnmtl, Dnmt3a, Jarid1b, Src1, Sirt1, Hdac1) were found between 30-day tissue cultures and age-matched in-vivo controls. DNMT1/DNMT3a expression and the presence of 5-methylcytosine (5mC) were detected in spermatogonia and leptotene/zygotene spermatocytes in cultures. The relative 5mC fluorescence intensity was similar in spermatozoa produced in cultures of cryopreserved tissues or in vivo. H3K4me3, H3K9ac and H4K8ac were present in all germ cell types but differences in the proportion of germ cells containing these epigenetic marks were found after cultures. Conclusions: Despite differences with the in-vivo situation, DNA methylation and histone methylation and acetylation occur in the mouse germline in in-vitro matured fresh or cryopreserved mouse prepubertal testicular tissue, and the expression of the enzymes catalysing these epigenetic modifications are maintained in vitro.

【 授权许可】

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