Receptors for thyroid-stimulating hormone and thyroid hormones in human ovarian tissue

REPRODUCTIVE BIOMEDICINE ONLINE

卷:18

Article

Aghajanova, Lusine¹  Lindeberg, Maria¹  Carlsson, Inger Britt¹  Stavreus-Evers, Anneli^1,2  Zhang, Pu¹  Scott, Jennifer E.¹  Hovatta, Outi¹  Skjoldebrand-Sparre, Lottie³ 

[1] Karolinska Univ, Huddinge Hosp, Karolinska Inst,Div Obstet & Gynaecol, Dept Clin Sci Intervent & Technol, S-14186 Huddinge, Sweden

[2] Uppsala Univ, Dept Womens & Childrens Hlth, Uppsala, Sweden

[3] Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Div Obstet & Gynaecol, S-18388 Stockholm, Sweden

关键词: cAMP;    ERK1/2;    granulosa cells;    human ovary;    thyroid hormone receptor;    thyroid stimulating hormone receptor;   

DOI  :  10.1016/S1472-6483(10)60091-0

来源: Elsevier

【 摘 要 】

Dysfunction in thyroid regulation can cause menstrual and ovulatory disturbances, the mechanism of which is not clear. The distribution and activity of the thyroid-stimulating hormone (TSHR), and the thyroid hormone receptors (TR) alpha 1, alpha 2 and beta 1 in human ovarian tissue and in granulosa cells was studied using immunohistochemistry, reverse-transcriptase polymerase chain reaction (RT-PCR), quantitative PCR and immunoassays. Strong immunostaining of TSHR, TR alpha 1 and TR beta 1 was observed in ovarian surface epithelium and in oocytes of primordial, primary and secondary follicles, with minimal staining in granulosa cells of secondary follicles. Granulosa cells of antral follicles expressed TSHR, TR alpha 1 and TR beta 1 proteins. Messenger RNA for all receptors was present in ovarian tissue. Mature human granulosa cells expressed transcripts for 5' deiodinases types 2 and 3, but not type 1, indicating the possibility of conversion of peripheral thyroid hormone thyroxin (T-4). Granulosa cells stimulated with TSH showed a significant increase in cAMP concentrations after 2 h of culture (P = 0.047), indicating activation through TSHR. Stimulation with T-4 resulted in increased extracellular signal-regulated kinase 1 and 2 activation after 10, 30, 60 min and 24 h. These data demonstrate that TSH and thyroid hormone receptors may participate in the regulation of ovarian function.

【 授权许可】

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