Identification of diabetes- and obesity-associated proteomic changes in human spermatozoa by difference gel electrophoresis

REPRODUCTIVE BIOMEDICINE ONLINE

卷:19

Article

Kriegel, Thomas M.²  Heidenreich, Falk²  Kettner, Karina²  Pursche, Theresia³  Hoflack, Bernard³  Grunewald, Sonja¹  Poenicke, Kerstin¹  Glander, Hans-Juergen¹  Paasch, Uwe¹ 

[1] Univ Leipzig, Dept Dermatol, Training Ctr, European Acad Androl, D-04103 Leipzig, Germany

[2] Tech Univ Dresden, Carl Gustav Carus Med Fac, Inst Physiol Chem, D-01307 Dresden, Germany

[3] Tech Univ Dresden, Ctr Biotechnol, Prote Grp, D-01307 Dresden, Germany

关键词: DIGE;    human spermatozoa;    obesity;    proteomics;    type 1 diabetes;   

DOI  :  10.1016/j.rbmo.2009.07.001

来源: Elsevier

【 摘 要 】

Difference gel electrophoresis (DIGE) of fluorescently labelled human sperm proteins was used to identify diabetes- and obesity-associated changes of the sperm proteome. Semen samples from type I diabetics, non-diabetic obese individuals and a reference group of clinically healthy fertile donors were evaluated in a comparative study. The adaptation of a general protein extraction procedure to the solubilization of proteins from isolated progressively motile human spermatozoa resulted in the detection of approximately 2700 fluorescent protein spots in the DIGE images. Comparison of the patients' sperm proteomes with those of the reference group allowed the identification of 20 spots containing proteins that were present in the sperm lysates at significantly increased or decreased concentrations. In detail, eight of these spots were apparently related to type I diabetes while 12 spots were apparently related to obesity. Tryptic digestion of the spot proteins and mass spectrometric analysis of the corresponding peptides identified seven sperm proteins apparently associated with type I diabetes and nine sperm proteins apparently associated with obesity, three of which existing in multiple molecular forms. The established proteomic approach is expected to function as a non-invasive experimental tool in the diagnosis of male infertility and in monitoring any fertility-restoring therapy.

【 授权许可】

Free