Identification and validation of candidate biomarkers involved in human ovarian autoimmunity

REPRODUCTIVE BIOMEDICINE ONLINE

卷:23

Article

Mande, Purvi V.¹  Parikh, Firuza R.^2,3  Hinduja, Indira⁴  Zaveri, Kusum⁴  Vaidya, Rama⁵  Gajbhiye, Rahul⁶  Khole, Vrinda V.¹ 

[1] Indian Council Med Res, Dept Gamete Immunobiol, Natl Inst Res Reprod Hlth, Bombay 400012, Maharashtra, India

[2] Jaslok Hosp, Dept Assisted Reprod & Genet, Bombay 400026, Maharashtra, India

[3] Res Ctr IVF Clin, Bombay 400026, Maharashtra, India

[4] INKUS IVF Clin, Bombay 400004, Maharashtra, India

[5] Kasturba Hlth Soc, Endocrinol Clin, Bombay 400056, Maharashtra, India

[6] Natl Inst Res Reprod Hlth, Dept Reprod Endocrinol & Infertil, Bombay 400012, Maharashtra, India

关键词: alpha-actinin 4;    beta-actin;    heat shock 70 protein 5;    IVF-embryo transfer;    ovarian autoimmunity;    POF;   

DOI  :  10.1016/j.rbmo.2011.06.013

来源: Elsevier

【 摘 要 】

Antibodies to multiple ovarian antigens have been proposed as markers of ovarian autoimmunity. The role of ovarian autoantibodies has been widely discussed in the pathophysiology of premature ovarian failure and unexplained infertility, but the autoantigens are yet to be identified. Three immunodominant ovarian autoantigens, alpha-actinin 4 (alpha ACTN4), heat shock 70 protein 5 (HSPA5) and beta-actin (ACTB), have been identified using anti-ovarian antibody-positive sera from women with idiopathic premature ovarian failure (n = 50) and women undergoing IVF (n = 695), using mass spectrometry. These autoantigens were subsequently validated using Western blot, immunohistochemistry and enzyme-linked immunosorbent assay. These autoantigens are localized to different components of the ovary such as the ooplasm of the oocyte, theca, granulosa, corpus luteum and zona pellucida. All the above antigens were found to be expressed in the ooplasm throughout follicular development. All the autoantigens are expressed specifically in the oocyte except alpha ACTN4. The three autoantigens could contribute to the array of biomarkers to be used for developing specific and sensitive tests for diagnosis of women at risk of premature ovarian failure and IVF failure due to ovarian autoimmunity and could give an insight into the molecular mechanisms involved in the pathophysiology of these conditions. (C) 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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