Elevated blood plasma antioxidant status is favourable for achieving IVF/ICSI pregnancy

REPRODUCTIVE BIOMEDICINE ONLINE

卷:26

Article

Velthut, Agne^1,2  Zilmer, Mihkel³  Zilmer, Kersti³  Kaart, Tanel⁴  Karro, Helle⁵  Salumets, Andres^2,5 

[1] Tallinn Univ Technol, Ctr Biol Integrated Syst, Tallinn, Estonia

[2] Competence Ctr Reprod Med & Biol, Tartu, Estonia

[3] Univ Tartu, Inst Biochem, Ctr Excellence Translat Med, EE-50090 Tartu, Estonia

[4] Estonian Univ Life Sci, Inst Vet Med & Anim Sci, Tartu, Estonia

[5] Univ Tartu, Dept Obstet & Gynaecol, EE-50090 Tartu, Estonia

关键词: antioxidative activity;    female infertility;    follicular fluid;    IVF;    ovarian stimulation;    oxidative stress;   

DOI  :  10.1016/j.rbmo.2012.12.012

来源: Elsevier

【 摘 要 】

The aim of the study was to determine the roles of intrafollicular and systemic oxidative stress and antioxidant response in ovarian stimulation and intracytoplasmic sperm injection (ICSI) outcomes. For this purpose, 102 ICSI patients undergoing controlled ovarian stimulation were enrolled and samples were collected on the day of follicle puncture. Total peroxide (TPX) concentrations and total antioxidant response (TAR) were measured in follicular fluid and blood plasma, and an oxidative stress index (OSI) was calculated based on these two parameters. Urinary concentrations of 8-iso-prostaglandin F-2a (F(2)IsoP) were measured. Elevated intrafollicular oxidative stress was positively correlated with ovarian stimulation outcome: less FSH per retrieved oocyte was used, more oocytes were collected and higher serum oestradiol concentrations were measured in patients with higher follicular OSI. However, high urinary F(2)IsoP related to lower embryo quality and F2IsoP was also elevated in smoking patients. Patients with endometriosis had lower follicular antioxidant status. Most importantly, higher systemic blood TAR was significantly favourable for achieving clinical pregnancy (P = 0.03). In conclusion, the findings suggest clear associations between oxidative stress, antioxidant status and several aspects of ovarian stimulation and IVF/ICSI outcome, including pregnancy rate. (C) 2013, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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