Cumulus cells of euploid versus whole chromosome 21 aneuploid embryos reveal differentially expressed genes

REPRODUCTIVE BIOMEDICINE ONLINE

卷:43

Article

Tiegs, Ashley W.^1,2  Titus, Shiny³  Mehta, Sameet⁴  Garcia-Milian, Rolando⁵  Seli, Emre^1,6  Scott, Richard T., Jr.^1,2 

[1] IVIRMA New Jersey, Basking Ridge, NJ 07920 USA

[2] Thomas Jefferson Univ, Dept Reprod Endocrinol & Infertil, Sidney Kimmel Med Coll, Philadelphia, PA 19107 USA

[3] Fdn Embryon Competence, Basking Ridge, NJ 07920 USA

[4] Yale Sch Med, Yale Ctr Genome Anal, New Haven, CT 06520 USA

[5] Yale Univ, Harvey Cushing John Hay Whitney Med Lib, Bioinformart Support Program, New Haven, CT 06520 USA

[6] Yale Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT 06510 USA

关键词: Aneuploid;    Cumulus cells;    Euploid;    Human embryos;    Transcriptome;   

DOI  :  10.1016/j.rbmo.2021.06.015

来源: Elsevier

【 摘 要 】

Research question: Can cumulus cells be used as a non-invasive target for the study of determinants of preimplantation embryo quality? Design: Cumulus cells were collected from monosomy 21, trisomy 21 and euploid embryos and subjected to RNA sequencing analysis and real-time polymerase chain reaction assays. The differential gene expression was analysed for different comparisons. Results: A total of 3122 genes in monosomy 21 cumulus cells and 19 genes in trisomy 21 cumulus cells were differentially expressed compared with euploid cumulus cells. Thirteen of these genes were differentially expressed in both monosomy and trisomy 21, compared with euploid, including disheveled segment polarity protein 2 (DVL2), cellular communication network factor 1 (CCN1/CYR61) and serum response factor (SRF), which have been previously implicated in embryo developmental competence. In addition, ingenuity pathway analysis revealed cell-cell contact function to be affected in both monosomy and trisomy 21 cumulus cells. Conclusions: These findings support the use of cumulus cell gene expression analysis for the development of biomarkers evaluating oocyte quality for patients undergoing fertility preservation of oocytes.

【 授权许可】

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