Biomethane production from whole and extracted algae biomass: Long-term performance evaluation and microbial community dynamics

RENEWABLE ENERGY

卷:170

Article

Ma, Jingwei¹  Li, Lu¹  Zhao, Quanbao²  Yu, Liang³  Frear, Craig⁴ 

[1] Hunan Univ, Coll Civil Engn, Dept Water Engn & Sci, Key Lab Bldg Safety & Energy Efficiency,Minist Ed, Changsha 410082, Hunan, Peoples R China

[2] Chinese Acad Sci, Inst Urban Environm, CAS Key Lab Urban Pollutant Convers, 1799 Jimei Rd, Xiamen 361021, Peoples R China

[3] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA

[4] Regenis Renewables & Environm, 6920 Salashan Pkwy,Suite A-102,POB 2708, Ferndale, WA 98248 USA

关键词: Anaerobic digestion;    Algal biomass;    Microbial community dynamics;    Organic loading rate;    Long-chain fatty acids;   

DOI  :  10.1016/j.renene.2021.01.113

来源: Elsevier

【 摘 要 】

Anaerobic digestion (AD) experiments were conducted on an industrial algae strain of Nannochloropsis salina in two identical 5-liter digesters for about 300 days. One digester was fed with whole cell algal biomass (WCB) while another with lipid-extracted algal biomass residues (LEB). WCB digester shows higher specific methane productivity ranging from 0.59 to 0.65 m(3) CH4/kgVS, while that in LEB digester ranges from 0.29 to 0.42 m(3) CH4/kgVS. Results show that organic loading rate (OLR) in LEB digester can reach up to 5.0 gVS/L/d, and WCB digester failed at that of 3.0 gVS/L/d since long-chain fatty acids (LCFA) were identified as the main inhibitor during AD of algal biomass. At these two maximum OLR, both WCB and LEB digester could achieve a volumetric methane production rate of 1.40 m(3) CH4/m(3)/d. Illumina MiSeq sequencing targeting 16S rRNA genes revealed that Proteiniclasticum, Tissierella, Methanosaeta and Methanomethylovorans were dominant genus at low OLR, while Trichococcus, Levilinea Methanosaeta and Methanobacterium dominated under high OLR. Furthermore, population shifts in methanogenic archaeal communities to Methanobacterium in WCB digester were noticed as aceticlastic methanogens are more sensitive to LCFA than hydrogenotrophic methanogens. (C) 2021 Elsevier Ltd. All rights reserved.

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