Valorization of Eucalyptus wood by glycerol-organosolv pretreatment within the biorefinery concept: An integrated and intensified approach

RENEWABLE ENERGY

卷:95

Article

Romani, Aloia¹  Ruiz, Hector A.²  Teixeira, Jose A.¹  Domingues, Lucilia¹ 

[1] Univ Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4710057 Braga, Portugal

[2] Autonomous Univ Coahuila, Sch Chem, Food Res Dept, Biorefinery Grp, Blvd V Carranza & Ing Jose Cardenas Valdes, Saltillo 25280, Coahuila, Mexico

关键词: High-gravity ethanol;    Organosolv pretreatment;    Lignocellulosic biomass;    Lignin characterization;    Biorefinery;    Industrial strain;   

DOI  :  10.1016/j.renene.2016.03.106

来源: Elsevier

【 摘 要 】

The efficient utilization of lignocellulosic biomass and the reduction of production cost are mandatory to attain a cost-effective lignocellulose-to-ethanol process. The selection of suitable pretreatment that allows an effective fractionation of biomass and the use of pretreated material at high-solid loadings on saccharification and fermentation (SSF) processes are considered promising strategies for that purpose. Eucalyptus globulus wood was fractionated by organosolv process at 200 degrees C for 69 min using 56% of glycerol-water. A 99% of cellulose remained in pretreated biomass and 65% of lignin was solubilized. Precipitated lignin was characterized for chemical composition and thermal behavior, showing similar features to commercial lignin. In order to produce lignocellulosic ethanol at high-gravity, a full factory design was carried to assess the liquid to solid ratio (3-9 g/g) and enzyme to solid ratio (8-16 FPU/g) on SSF of delignified Eucalyptus. High ethanol concentration (94 g/L) corresponding to 77% of conversion at 16FPU/g and LSR = 3 g/g using an industrial and thermotolerant Saccharomyces cerevisiae strain was successfully produced from pretreated biomass. Process integration of a suitable pretreatment, which allows for whole biomass valorization, with intensified saccharification-fermentation stages was shown to be feasible strategy for the co-production of high ethanol titers, oligosaccharides and lignin paving the way for cost-effective Eucalyptus biorefinery. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

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