Docosahexaenoic acid confers enduring neuroprotection in experimental stroke

JOURNAL OF THE NEUROLOGICAL SCIENCES

卷:338

Article

Hong, Sung-Ha¹  Belayev, Ludmila^1,2  Khoutorova, Larissa¹  Obenaus, Andre³  Bazan, Nicolas G.¹ 

[1] Louisiana State Univ, Hlth Sci Ctr, Neurosci Ctr Excellence, New Orleans, LA 70112 USA

[2] Louisiana State Univ, Hlth Sci Ctr, Dept Neurosurg, New Orleans, LA 70112 USA

[3] Loma Linda Univ, Noninvas Imaging Lab, Loma Linda, CA 92350 USA

关键词: Docosahexaenoic acid;    Experimental stroke;    Middle cerebral artery occlusion;    Behavior;    Rota-rod test;    Beam walking test;    Y maze test;    Ex vivo MRI;   

DOI  :  10.1016/j.jns.2013.12.033

来源: Elsevier

【 摘 要 】

Recently we demonstrated that docosahexaenoic acid (DHA) is highly neuroprotective when animals were allowed to survive during one week. This study was conducted to establish whether the neuroprotection induced by DHA persists with chronic survival. Sprague-Dawley rats underwent 2 h of middle cerebral artery occlusion (MCAo) and treated with DHA or saline at 3 h after MCAo. Animals received neurobehavioral examination (composite neuroscore, rota-rod, beam walking and Y maze tests) followed by ex vivo magnetic resonance imaging and histopathology at 3 weeks. DHA improved composite neurologic score beginning on day 1 by 20%, which persisted throughout weeks 1-3 by 24-41% compared to the saline-treated group. DHA prolonged the latency in rota-rod on weeks 2-3 by 162-178%, enhanced balance performance in the beam walking test on weeks 1 and 2 by 42-51%, and decreased the number of entries in the Y maze test by 51% and spontaneous alteration by 53% on week 2 compared to the saline-treated group. DHA treatment reduced tissue loss (computed from T2-eighted images) by 24% and total and cortical infarct volumes by 46% and 54% compared to the saline-treated group. These results show that DHA confers enduring ischemic neuroprotection. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.

【 授权许可】

Free