Decreased peripheral nerve damage after ischemia-reperfusion injury in mice lacking TNF-alpha

JOURNAL OF THE NEUROLOGICAL SCIENCES

卷:267

Article

Wang, Yanping¹  Kawamura, Nobutoshi¹  Schmelzer, James D.¹  Schmeichel, Ann M.¹  Low, Phillip A.¹ 

[1] Mayo Clin, Dept Neurol, Rochester, MN 55905 USA

关键词: ischemia-reperfusion (IR) injury;    peripheral nerve;    tumor necrosis factor-alpha (TNF-alpha);    nuclear factor-kappa B (NF-kappa B);   

DOI  :  10.1016/j.jns.2007.10.004

来源: Elsevier

【 摘 要 】

We sought to explore the role of tumor necrosis factor-alpha (TNF-alpha) in the pathogenesis of peripheral nerve ischemia-reperfusion (IR) injury. We established an ischemia-reperfusion model in wild type (WT) and TNF-alpha knockout (KO) mice. Electrophysiology, behavioral score and morphological indices (edema and ischemic fiber degeneration [IFD]) were examined to determine the influence of TNF-alpha on peripheral nerve structure and function following ischemia followed by reperfusion. TNF-alpha and nuclear factor-kappa B (NF-kappa B) expression wereevaluated using immunohistochemistry. TNF-alpha KO mice, compared to WT had, in sciatic nerve, marked improvement in nerve pathology. This is a region subject to moderate ischemia-reperfusion injury. There was also a significant improvement in electrophysiological and some behavioral indices. TNF-alpha and NF-kappa B expression were abundant in sciatic-tibial nerves of WT mice subjected to IR, but there was less, or complete lack of, expression in ischemic nerve of TNF-alpha KO mice. We conclude that TNF-alpha plays an essential role in the pathogenesis of peripheral nerve ischemia-reperfusion injury, possibly partly through the activation of NF-kappa B. (C) 2007 Elsevier B.V. All rights reserved.

【 授权许可】

Free