Epicardial transplantation of atrial appendage micrograft patch salvages myocardium after infarction

JOURNAL OF HEART AND LUNG TRANSPLANTATION

卷:39

Article

Yanbo, Xie¹  Lampinen, Milla²  Takala, Juuso²  Sikorski, Vilbert²  Soliymani, Rabah^3,4  Tarkia, Miikka²  Lalowski, Maciej^3,4  Mervaala, Eero²  Kupari, Markku^5,6  Zheng, Zhe¹  Hu, Shengshou¹  Harjula, Ari^5,6  Kankuri, Esko² 

[1] Chinese Acad Med Sci & Peking Union Med Coll, Natl Ctr Cardiovasc Dis, Fuwai Hosp, State Key Lab Cardiovasc Dis, Beijing, Peoples R China

[2] Univ Helsinki, Dept Pharmacol, Fac Med, Haartmaninkatu 8,POB 63, FIN-00014 Helsinki, Finland

[3] Univ Helsinki, Dept Biochem & Dev Biol, Fac Med, Helsinki, Finland

[4] Univ Helsinki, Helsinki Inst Life Sci HiLIFE, Helsinki, Finland

[5] Helsinki Univ Cent Hosp, Heart & Lung Ctr, Helsinki, Finland

[6] Univ Helsinki, Helsinki, Finland

关键词: cell therapy;    myocardial infarction;    heart failure;    myocardial regeneration;    atrial appendage;    site-selective proteomics;   

DOI  :  10.1016/j.healun.2020.03.023

来源: Elsevier

【 摘 要 】

BACKGROUND: Ischemic heart disease remains the leading cause of mortality and morbidity worldwide despite improved possibilities in medical care. Alongside interventional therapies, such as coronary artery bypass grafting, adjuvant tissue-engineered and cell-based treatments can provide regenerative improvement. Unfortunately, most of these advanced approaches require multiple lengthy and costly preparation stages without delivering significant clinical benefits. METHODS: We evaluated the effect of epicardially delivered minute pieces of atrial appendage tissue material, defined as atrial appendage micrografts (AAMs), in a mouse myocardial infarction model. An extracellular matrix patch was used to cover and fix the AAMs onto the surface of the infarcted heart. RESULTS: The matrix-covered AAMs salvaged the heart from the infarction-induced loss of functional myocardium and attenuated scarring. Site-selective proteomics of injured ischemic and uninjured distal myocardium from AAMs-treated and -untreated tissue sections revealed increased expression of several cardiac regeneration-associated proteins (i.e., periostin, transglutaminases, and glutathione peroxidases) and activation of pathways responsible for angiogenesis and cardiogenesis in relation to AAMs therapy. CONCLUSIONS: Epicardial delivery of AAMs encased in an extracellular matrix patch scaffold salvages functional cardiac tissue from ischemic injury and restricts fibrosis after myocardial infarction. Our results support the use of AAMs as tissue-based therapy adjuvants for salvaging the ischemic myocardium. (C) 2020 The Author(s). Published by Elsevier Inc. on behalf of International Society for Heart and Lung Transplantation. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)

【 授权许可】

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