Signaling pathways associated with macrophage-activating polysaccharide isolated from the fermentation liquor of Rhizopus nigricans

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:30

Article

Yu, Zhidan^1,2  Li, Xiaoqin¹  Zhou, Fang¹  Zhang, Pengying²  Chen, Kaoshan^2,3 

[1] Zhengzhou Univ, Henan Childrens Hosp, Zhengzhou Childrens Hosp,Childrens Hosp, Dept Gastroenterol,Henan Prov Key Lab Childrens G, Zhengzhou 450018, Peoples R China

[2] Shandong Univ, Sch Life Sci, 72 Binhai Rd, Qingdao 266000, Peoples R China

[3] Shandong Univ, Natl Glycoengn Rechearch Ctr, 72 Binhai Rd, Qingdao 266000, Peoples R China

关键词: Polysaccharide;    Rhizopus nigricans;    TLR4;    MAPK;    NF-kappa B;   

DOI  :  10.1016/j.bmcl.2020.127297

来源: Elsevier

【 摘 要 】

Our previous reports showed that the structural features and immunologic enhancement of polysaccharide (EPS1-1) from Rhizopus nigricans. However, the molecular mechanism in cellular immunomodulatory of EPS1-1 remains unclear. Here the experiments for the molecular mechanisms of EPS1-1 on the peritoneal macrophages were performed. The results demonstrated that the expression of TLR4 was significantly improved by EPS1-1. Subsequently, the phosphorylation of p38MAPK, ERK1/2, JNK and IKKa/13 were promoted. Moreover, EPS1-1 enhanced the expressions of IL-2, TNF-alpha and iNOS in EPS1-1-induced macrophages which were pretreated with MAPK signaling pathway inhibitors, and reduced the blocking effects of the inhibitors to the expressions of p-p38MAPK, p-ERK1/2 and p-IKK alpha/beta. Therefore, these results illustrated that EPS1-1 could improve the immune functions of peritoneal macrophages by promoting the gene expressions of IL-2, TNF-alpha and iNOS via the MAPK and NF-kappa B signaling pathways.

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