Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:20

Article

Liu, Jia¹  Castaneda, Carlos A.¹  Wilkins, Bryan J.¹  Fushman, David¹  Cropp, T. Ashton¹ 

[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

关键词: Unnatural amino acid;    tRNA;    Ubiquitin;    Aminoacyl-tRNA synthetase;   

DOI  :  10.1016/j.bmcl.2010.08.049

来源: Elsevier

【 摘 要 】

Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures. (C) 2010 Elsevier Ltd. All rights reserved.

【 授权许可】

Free