Cell surface glycoproteomic analysis of prostate cancer-derived PC-3 cells

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:21

Article

Hubbard, Sarah C.¹  Boyce, Michael¹  McVaugh, Cheryl T.¹  Peehl, Donna M.³  Bertozzi, Carolyn R.^1,2 

[1] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Stanford Univ, Sch Med, Dept Urol, Stanford, CA 94305 USA

关键词: Metabolic labeling;    Glycoproteomics;    Prostate cancer;    Azidosugar;    Tissue slice cultures;   

DOI  :  10.1016/j.bmcl.2011.05.045

来源: Elsevier

【 摘 要 】

Most clinically approved biomarkers of cancer are glycoproteins, and those residing on the cell surface are of particular interest in biotherapeutics. We report a method for selective labeling, affinity enrichment, and identification of cell-surface glycoproteins. PC-3 cells and primary human prostate cancer tissue were treated with peracetylated N-azidoacetylgalactosamine, resulting in metabolic labeling of cell surface glycans with the azidosugar. We used mass spectrometry to identify over 70 cell surface glycoproteins and biochemically validated CD146 and integrin beta-4, both of which are known to promote metastatic behavior. These results establish cell-surface glycoproteomics as an effective technique for discovery of cancer biomarkers. (C) 2011 Published by Elsevier Ltd.

【 授权许可】

Free