New monoterpene glycosides and phenolic compounds from Distylium racemosum and their inhibitory activity against ribonuclease H

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:21

Article

Kim, Jeong Ah¹  Yang, Seo Young¹  Wamiru, Anthony^2,3  McMahon, James B.²  Le Grice, Stuart F. J.⁴  Beutler, John A.²  Kim, Young Ho^1,2 

[1] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea

[2] NCI, Mol Targets Lab, Ctr Canc Res, Frederick, MD 21702 USA

[3] NCI, SAIC Frederick, Ctr Canc Res, Frederick, MD 21702 USA

[4] NCI, HIV Drug Resistance Program, Ctr Canc Res, Frederick, MD 21702 USA

关键词: Distylium racemosum;    Monoterpene glycosides;    Megastigmane glucoside;    Ribonuclease H;   

DOI  :  10.1016/j.bmcl.2011.03.091

来源: Elsevier

【 摘 要 】

Two new monoterpene glycosides, distyloside A-B (1-2), and a new megastigmane glucoside, iso-dihydrodendranthemoside A (3) were isolated from twigs and leaves of Distylium racemosum, along with five known phenolic compounds (4-8). The structures were established via spectroscopic techniques and chemical transformations, and the absolute stereochemistry of 3 was determined by Mosher's esterification. A homogeneous fluorescence resonance energy transfer (FRET) quenching assay was used to determine the inhibitory activity of isolates (1-8) on the ribonuclease H enzymes from HIV-1, 2, human, and Escherichia coli. Among them, 6 ''-O-galloylsalidroside (6) showed potent inhibitory effects with an IC(50) value of 3.5 mu M on HIV-2, and 1.7 mu M on human RNase H, respectively. (C) 2011 Elsevier Ltd. All rights reserved.

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