Facile conversion of RNA aptamers to modular fluorescent sensors with tunable detection wavelengths

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:21

Article

Nakano, Shun^1,2  Nakata, Eiji¹  Morii, Takashi^1,2 

[1] Kyoto Univ, Inst Adv Energy, Kyoto 6110011, Japan

[2] JST, CREST, Kyoto 6110011, Japan

关键词: Aptamer;    Ribonucleopeptide;    Biosensor;    Modular design;    Fluorescence detection;   

DOI  :  10.1016/j.bmcl.2011.05.120

来源: Elsevier

【 摘 要 】

A GTP aptamer was converted to a modular fluorescent GTP sensor by conjugation of RRE (Rev responsive element) RNA and successive complex formation with a fluorophore-modified Rev peptide. Structural changes associated with substrate binding in the RNA aptamer were successfully transduced into changes in fluorescence intensity because of the modular structure of ribonucleopeptides. A simple modular strategy involving conjugation of a fluorophore-modified ribonucleopeptide to the stem region of an RNA aptamer deduced from secondary structural information helps produce fluorescent sensors, which allow tuning of excitation and detection wavelengths through the replacement of the fluorophore at the N-terminal of the Rev peptide. (C) 2011 Elsevier Ltd. All rights reserved.

【 授权许可】

Free