BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 卷:21 |
Toward self-adjuvanting subunit vaccines: Model peptide and protein antigens incorporating covalently bound toll-like receptor-7 agonistic imidazoquinolines | |
Article | |
Shukla, Nikunj M.1  Lewis, Tyler C.1  Day, Timothy P.1  Mutz, Cole A.1  Ukani, Rehman1  Hamilton, Chase D.1  Balakrishna, Rajalakshmi1  David, Sunil A.1  | |
[1] Univ Kansas, Dept Med Chem, Lawrence, KS 66047 USA | |
关键词: Imidazoquinoline; Toll-like receptor; TLR7; Self-adjuvanting vaccine; ELISA; Adjuvant; | |
DOI : 10.1016/j.bmcl.2011.04.050 | |
来源: Elsevier | |
【 摘 要 】
Toll-like receptor (TLR)-7 agonists show prominent Th1-biased immunostimulatory activities. A TLR7-active N1-(4-aminomethyl) benzyl substituted imidazoquinoline 1 served as a convenient precursor for the syntheses of isothiocyanate and maleimide derivatives for covalent attachment to free amine and thiol groups of peptides and proteins. 1 was also amenable to direct reductive amination with maltoheptaose without significant loss of activity. Covalent conjugation of the isothiocyanate derivative 2 to alpha-lactalbumin could be achieved under mild, non-denaturing conditions, in a controlled manner and with full preservation of antigenicity. The self-adjuvanting alpha-lactalbumin construct induced robust, high-affinity immunoglobulin titers in murine models. The premise of covalently decorating protein antigens with adjuvants offers the possibility of drastically reducing systemic exposure of the adjuvant, and yet eliciting strong, Th1-biased immune responses. (C) 2011 Elsevier Ltd. All rights reserved.
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