2-(Nitroaryl)benzothiazole and benzoxazole derivatives as fluorogenic substrates for the detection of nitroreductase activity in clinically important microorganisms

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:25

Article

Cellier, Marie¹  Gignoux, Amandine^2,3  James, Arthur L.²  Orenga, Sylvain¹  Perry, John D.⁴  Robinson, Shaun N.²  Stanforth, Stephen P.²  Turnbull, Graeme² 

[1] BioMerieux SA, Res & Dev Microbiol, F-38390 La Balme Les Grottes, France

[2] Northumbria Univ, Dept Appl Sci, Newcastle Upon Tyne NE1 8ST, Tyne & Wear, England

[3] Ecole Natl Super Chim Paris, F-75231 Paris 05, France

[4] Freeman Rd Hosp, Dept Microbiol, Newcastle Upon Tyne NE7 7DN, Tyne & Wear, England

关键词: Nitroreductase;    Fluorescence;    Enzyme substrates;    Microorganism detection;    Bacterial detection;   

DOI  :  10.1016/j.bmcl.2015.10.099

来源: Elsevier

【 摘 要 】

A series of carboxy-substituted 2-(nitroaryl)benzothiazole derivatives and carboxy-substituted 2-(nitroaryl)benzoxazole derivatives were prepared and evaluated as potential nitroreductase substrates for the purpose of detecting clinically important microorganisms. Several of the substrates produced highly fluorescent colonies with the majority of a panel of 10 Gram-negative bacteria and also with two of a panel of 8 Gram-positive bacteria. (C) 2015 Elsevier Ltd. All rights reserved.

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