Multivalent LKγ-PNA oligomers bind to a human telomere DNA G-rich sequence to form quadruplexes

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:25

Article

Gupta, Pankaj¹  Rastede, Elizabeth E.¹  Appella, Daniel H.¹ 

[1] NIDDK, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA

关键词: Quadruplex;    DNA;    PNA;   

DOI  :  10.1016/j.bmcl.2015.07.075

来源: Elsevier

【 摘 要 】

We report G-quadruplex formation between peptide nucleic acids (PNAs) composed of K-L gamma-PNA-G monomers and a known portion of human telomeric DNA that adopts three G3 tracts via intramolecular hydrogen bonding. The resulting complex is a bimolecular PNA-DNA heteroquadruplex. In this Letter, we show that introduction of a gamma-modification and addition of a peptide ligand does not disrupt the heteroquadruplex. Although the unmodified PNA1 forms a quadruplex with itself, the gamma-substituted PNAs (PNA2-PNA6) do not form G-quadruplexes on their own, at even high concentrations. The selectivity of these PNAs could influence the design of new quadruplex-targeting molecules or allow the quadruplex structure to be used as a scaffold for multivalent display of protein binding ligands. Published by Elsevier Ltd.

【 授权许可】

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