Approaches to the simultaneous inactivation of metallo- and serine-β-lactamases

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:19

Article

Ganta, Sudhakar Reddy¹  Perumal, Senthil²  Pagadala, Sundar Ram Reddy¹  Samuelsen, Orjan^3,4  Spencer, James³  Pratt, R. F.²  Buynak, John D.¹ 

[1] So Methodist Univ, Dept Chem, Dallas, TX 75275 USA

[2] Wesleyan Univ, Dept Chem, Middletown, CT 06459 USA

[3] Univ Bristol, Sch Med Sci, Dept Cellular & Mol Med, Bristol BS8 1TD, Avon, England

[4] Univ Hosp N Norway, Dept Microbiol & Infect Control, Reference Ctr Detect Antimicrobial Resistance, N-9038 Tromso, Norway

关键词: beta-Lactamase;    Antibiotic;    Penicillin;    Cephalosporin;    Hydroxamate;    Reverse hydroxamate;   

DOI  :  10.1016/j.bmcl.2009.02.018

来源: Elsevier

【 摘 要 】

A series of cephalosporin-derived reverse hydroxamates and oximes were prepared and evaluated as inhibitors of representative metallo-and serine-beta-lactamases. The reverse hydroxamates showed submicromolar inhibition of the GIM-1 metallo-beta-lactamase. With respect to interactions with the classes A, C, and D serine beta-lactamases, as judged by their correspondingly low K-m values, the reverse hydroxamates were recognized in a manner similar to the non-hydroxylated N-H amide side chains of the natural substrates of these enzymes. This indicates that, with respect to recognition in the active site of the serine b-lactamases, the O=C-NR-OH functionality can function as a structural isostere of the O=C-NR-H group, with the N-O-H group presumably replacing the amide N-H group as a hydrogen bond donor to the appropriate backbone carbonyl oxygen of the protein. The reverse hydroxamates, however, displayed k(cat) values up to three orders of magnitude lower than the natural substrates, thus indicating substantial slowing of the hydrolytic action of these serine beta-lactamases. Although the degree of inactivation is not yet enough to be clinically useful, these initial results are promising. The substitution of the amide N-H bond by N-OH may represent a useful strategy for the inhibition of other serine hydrolases. Published by Elsevier Ltd.

【 授权许可】

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