Opposing effects of cannabinoids and vanilloids on evoked quantal release at the frog neuromuscular junction

NEUROSCIENCE LETTERS

卷:473

Article

Silveira, Priscila Elisa¹  Silveira, Naiara Araujo¹  Morini, Veronica de Cassia¹  Kushmerick, Christopher¹  Naves, Ligia Araujo¹ 

[1] Univ Fed Minas Gerais, ICB, Dept Fisiol & Biofis, BR-31270901 Belo Horizonte, MG, Brazil

关键词: Neuromuscular junction;    Cannabinoid;    Vanilloid;    Acetylcholine;   

DOI  :  10.1016/j.neulet.2010.02.026

来源: Elsevier

【 摘 要 】

Cannabinoids and vanilloids are two distinct groups of substances that share some pharmacological targets. Here we report that two cannabinoid type 1 receptor (CB1) agonists, WIN 55212-2 (WIN) and arachidonyl-2'-chloroethylamide (ACEA) have opposing effects on evoked quantal acetylcholine release - WIN decreased quantal content while ACEA increased quantal content. The decrease in quantal content by WIN was blocked by the CBI antagonist AM 251. The increase in quantal content by ACEA was not blocked by AM 251, indicating it acts through a receptor other than CBI. As ACEA is also an agonist for the vanilloid receptor (TRPV1) we tested the effect of vanilloids on quantal content. Similar to ACEA, the vanilloid agonist capsaicin increased quantal content, and this effect was blocked by capsazepine, a TRPV1 antagonist. Capsazepine also blocked the increase in quantal content by ACEA. Together these data show an inhibitory effect of CB1 activation on evoked acetylcholine release and the first evidence for the presence of a vanilloid receptor at the neuromuscular junction. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free