Central antioxidant therapy inhibits parasympathetic baroreflex control in conscious rats

NEUROSCIENCE LETTERS

卷:489

Article

Braga, Valdir Andrade²  Colombari, Eduardo¹ 

[1] Fac Med ABC, Dept Physiol, Sch Med ABC, BR-09060870 Santo Andre, SP, Brazil

[2] Univ Fed Paraiba, Pharmaceut Technol Lab, BR-58059900 Joao Pessoa, Paraiba, Brazil

关键词: Baroreceptor reflex;    Ascorbic acid;    N-acetylcysteine;    Parasympathetic nervous system;    Reactive oxygen species;    Brainstem;   

DOI  :  10.1016/j.neulet.2010.11.077

来源: Elsevier

【 摘 要 】

Baroreceptor reflex is an important system for neural control of blood pressure. Recently, reactive oxygen species (ROS) have been shown to play an important role in neuronal activity of central areas related to blood pressure control. The aim of this study was to investigate the effects elicited by ascorbic acid (AAC) and N-acetylcysteine (NAC) injections into the 4thV on the parasympathetic component of the baroreflex. Male Wistar rats were implanted with a stainless steel guide cannula into the 4thV. One day prior to the experiments, the femoral artery and vein were cannulated for pulsatile arterial pressure, mean arterial pressure and heart rate measurements and drug administration, respectively. After baseline recordings, the baroreflex was tested with a pressor dose of phenylephrine (PHE, 3 mu g/kg, i.v.) and a depressor dose of sodium nitroprusside (SNP, 30 mu g/kg, i.v.) before (control) and 5, 15,30 and 60 min after AAC or NAC into the 4thV. Control PHE injection induced baroreflex-mediated bradycardia (-93 +/- 13 bpm, n = 7). Interestingly, after AAC injection into the 4thV, PHE injection produced a transient tachycardia at 5 (40 +/- 23 bpm), 15 (26 +/- 22 bpm) and 30 min (59 +/- 21 bpm). No changes were observed in baroreflex-mediated tachycardia evoked by SNP after AAC injection on 4thV (control: 151 +/- 23 bpm vs. 135 +/- 18 bpm at 5 min after MC, n = 7). In the NAC treated group. PHE induced a reduction in reflex bradycardia at 5 min when compared to control (-11 +/- 17 bpm vs. -83 +/- 15 bpm, n = 7). No changes were observed in baroreflex-mediated tachycardia evoked by SNP after NAC injection on 4thV. The antioxidants AAC and NAC may act in the central nervous system affecting the parasympathetic component of the cardiac baroreflex. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

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