C-FOS RESPONSE TO ADMINISTRATION OF CATECHOLAMINES INTO BRAIN BY MICRODIALYSIS

NEUROSCIENCE LETTERS

卷:133

Article

STONE, EA ; ZHANG, Y ; JOHN, SM ; BING, GY

关键词: C-FOS;    MICRODIALYSIS;    CATECHOLAMINE;    NE;    IMMUNOHISTOCHEMISTRY;   

DOI  :  10.1016/0304-3940(91)90050-4

来源: Elsevier

【 摘 要 】

The present studies investigated the use of microdialysis for the infusion of catecholamines into the brain in studies of immediate early gene (IEG) activation. c-Fos like immunoreactivity was examined histochemically in cortical tissue surrounding a dialysis probe implanted in the medial prefrontal cortex. No c-fos reactivity was observed at 48 h post implantation in control animals not probe-infused or infused 2 h earlier with Ringer's buffer. Marked reactivity was found in animals infused with a solution of norepinephrine (NE). The latter was blocked by coinfusion of the beta adrenoceptor antagonist, timolol. It is concluded that direct infusion of NE by dialysis can induce c-fos expression in cells of the cerebral cortex and that this effect is primarily via beta-adrenoceptors. Microdialysis therefore represents a useful technique in studies of IEG responses to brain catecholamines.

【 授权许可】

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