Obligatory role of ASK1 in the apoptotic surge of K+ currents

NEUROSCIENCE LETTERS

卷:387

Article

Aras, MA ; Aizenman, E

关键词: apoptosis signal-regulating kinase 1;    potassium channels;    oxidative stress;    cortical neuron;    thioredoxin;   

DOI  :  10.1016/j.neulet.2005.06.024

来源: Elsevier

【 摘 要 】

Apoptosis signal-regulating kinase I (ASK I) is a critical component of mitogen-activated protein kinase signaling pathways leading to cell death in response to cytokines and cellular stress. We use a dominant-negative (DN) form of ASK1 to show that this enzyme is necessary for the delayed surge in neuronal K+ channel activity, a required step in apoptosis. Furthermore, expression of ASK1 DN also suppresses the apoptotic increase in Kv2.1 currents transiently expressed in Chinese hamster ovary cells. Finally, over-expression of thioredoxin, an inhibitory binding partner of ASK1, is sufficient to halt the apoptotic current surge in neurons. Thus, ASK1 is an obligatory component of the pro-apoptotic modulation of K+ channels. (C) 2005 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free