Changes in intramuscular cytokine levels during masseter inflammation in male and female rats

NEUROSCIENCE LETTERS

卷:487

Article

Niu, Katelyn Y.¹  Ro, Jin Y.¹ 

[1] Univ Maryland Baltimore, Sch Dent, Dept Neural & Pain Sci, Baltimore, MD 21201 USA

关键词: Complete Freund's adjuvant;    TNF-alpha, IL-1 beta;    IL-6;    IL-4;    Sex;    Muscle;   

DOI  :  10.1016/j.neulet.2010.10.027

来源: Elsevier

【 摘 要 】

The present study was conducted to examine cytokine profiles in the masseter muscle before and after complete Freund's adjuvant (CFA)-induced inflammation and possible sex differences in the cytokine levels. Age matched male and female Sprague Dawley rats were injected with CFA in the mid-region of the masseter muscle. Muscle tissue surrounding the injection site was extracted 6 h, 1,3 and 7 days after the injection to measure TNF-alpha, IL-1 beta, IL-6 and IL-4 levels with Luminex multi-analyte profiling (xMAP) technology. The cytokine levels were compared to those obtained from naive rats. CFA injection into the masseter muscle led to a significant time effect in the level of TNF-a compared to that of naive rats. The pattern of changes in TNF-alpha level after CFA injection was significantly different between the male and female rats owing to the differences in basal levels. CFA injection induced significant time-dependent increases in the levels of IL-1 beta and IL-6 in the masseter muscle in both male and female rats. The level of IL-4 was slightly, but significantly, reduced in both sexes at 6 hand 3 days after CFA-induced inflammation. No significant sex differences were observed in the levels of IL-1 beta, IL-6 or IL-4. The results provided novel information about distinct cytokine profiles during CFA-induced muscle inflammation, and the basis for further pursuing contributions of each cytokine in pain processing and analgesic responses in both sexes. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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